Maternal N‐acetyl‐cysteine prevents neonatal brain injury associated with necrotizing enterocolitis in a rat model
نویسندگان
چکیده
Introduction Preterm infants with necrotizing enterocolitis (NEC) are at increased risk of cerebral injury and neurodevelopmental dysfunction. N-acetyl-cysteine (NAC) is a known anti-inflammatory antioxidant agent. Currently, there no prophylactic treatment in clinical use to prevent NEC its sequelae. We sought determine whether brain inflammation/apoptosis accompanies systemic inflammation, it can be attenuated by maternal NAC during pregnancy and/or the neonatal period rat model. Material methods An established newborn model (hypoxia 5% O2 for 10 min formula feeding thrice daily, beginning on day 1 4 days) was used Sprague-Dawley pups (n = 32). additional group 33) received (300 mg/kg intraperitoneal daily) addition conditions (NEC-NAC). Control were nursed remained dam room air. Two groups included dams treated once daily intravenous) last 3 days pregnancy. After birth, randomized into NAC-NEC NAC-NEC-NAC 36) postnatal treatment. Pups sacrificed fifth life. Pup serum interleukin (IL)-6 protein levels, nuclear factor kappa B (NF-κB) p65, neuronal nitric oxide synthase (nNOS), Caspase 3, tumor necrosis alpha (TNF-α), IL-6 IL-1β levels determined ELISA, western blot TUNEL staining, compared using analysis variance (ANOVA). Results had significantly control as well apoptosis NF-κB, nNOS, TNF-α, control. In all groups, IL-6, reduced group. The most pronounced decrease demonstrated within Conclusions attenuate inflammatory response syndrome offspring neuroapoptosis inflammation inhibition nNOS pathways.
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ژورنال
عنوان ژورنال: Acta Obstetricia et Gynecologica Scandinavica
سال: 2021
ISSN: ['0001-6349', '1600-0412']
DOI: https://doi.org/10.1111/aogs.14054